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Agilent 1200 Series G4226A 1290 Infinity Autosampler

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Original price was: $12,900.00.Current price is: $6,400.00.


Agilent 1200 Series G4226A 1290 Infinity Autosampler offers a comprehensive portfolio of LC solutions that give you uncompromised chromatographic performance. Agilent instruments, supplies, and critical instrument parts are essential for confidence in your results.


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Agilent 1200 Series G4226A 1290 Infinity Autosampler

Agilent 1200 Series G4226A 1290 Infinity Autosampler offers a comprehensive portfolio of LC solutions that give you uncompromised chromatographic performance. Agilent instruments, supplies, and critical instrument parts are essential for confidence in your results.

The 1290 Infinity Autosampler is part of the 1290 Infinity LC system that is compatible with operating pressures up to 1200 bar. It features the next-generation flow-through design for high precision injection of both large and small volumes without the need to change sample loops.

The 1290 Infinity Autosampler features an increased pressure range
enabling the use of today’s column technology (sub- two- micron narrow
bore columns) with the Agilent 1290 Infinity LC System. Increased
robustness is achieved by optimized new parts, high speed with lowest
carry- over by flow through design, increased sample injection speed for
high sample throughput, increased productivity by using overlapped
injection mode and flexible and convenient sample handling with different
types of sample containers, such as vials and well plates. Using 384- well
plates allows you to process up to 768 samples unattended.

Overview of the Module
The Autosampler transport mechanism uses an X- Z- theta robot to
optimize the positioning of the sampling arm on the well plate. Once the
sampling arm is positioned over the programmed sample position, the
programmed sample volume is drawn by the metering device into the
sampling needle. The sampling arm then moves to the injection position
where the sample is flushed onto the column.
The autosampler employ a vial/plate pusher mechanism to hold down the
vial or the plate while the needle is drawn back from the sample vessel (a
must in the case a septum is used). This vial/plate pusher employs a
sensor to detect the presence of a plate and to ensure accurate movement
regardless of plate used. All axes of the transport mechanism
(x- ,z- ,theta- robot) are driven by stepper- motors. Optical encoders ensure
the correct operation of the movement.
The standard metering device provides injection volumes from 0.1–20 µl. A
0.1- 40 µl injection volume metering device is installed in the G4226A, with
a 20 µl, low restriction loop capillary restricting the injection volume. The
entire flowpath including the metering device is always flushed by the
mobile phase after injection for minimum internal carry- over.
An additional needle flush station with a peristaltic pump is installed to
wash the outside of the needle. This reduces the already low carry- over
for very sensitive analysis.
The bottle containing the mobile phase for the wash procedure will be
located in the solvent bottle cabinet. Produced waste during this operation
is channeled safely away through a waste drain.
The six- port (only 5 ports are used) injection valve unit is driven by a
high- speed hybrid stepper motor. During the sampling sequence, the valve
unit bypasses the autosampler, and connects flow from the pump to the
column directly. During injection and analysis, the valve unit directs the
flow through the autosampler which ensures that all of the sample is
injected onto the column, and that the metering unit and needle are
always free from sample residue before the next sampling sequence begins

Overview of the Module
Control of the vial/plate temperature in the thermostatted autosampler is
achieved using an additional Agilent 1200 Series module; the Agilent 1200
Series thermostat for ALS/FC/Spotter. The thermostat contains
Peltier- controlled heat- exchangers. A fan draws air from the area above
the sample vial tray of the autosampler. It is then blown through the fins
of the cooling/heating module. There it is cooled or heated according the
temperature setting. The thermostatted air enters the autosampler through
a recess underneath the special designed sample tray. The air is then
distributed evenly through the sample tray ensuring effective temperature
control, regardless of how many vials are in the tray. In cooling mode
condensation is generated on the cooled side of the Peltier elements. This
condensed water is safely guided into a waste bottle for condensed water.

Autosampler Principle
The movements of the autosampler components during the sampling
sequence are monitored continuously by the autosampler processor. The
processor defines specific time windows and mechanical ranges for each
movement. If a specific step of the sampling sequence is not completed
successfully, an error message is generated. Solvent is bypassed from the
autosampler by the injection valve during the sampling sequence. The
needle moves to the desired sample position and is lowered into the
sample liquid in the sample to allow the metering device to draw up the
desired volume by moving its plunger back a certain distance. The needle
is then raised again and moved onto the seat to close the sample loop.
Sample is applied to the column when the injection valve returns to the
mainpass position at the end of the sampling sequence.
The standard sampling sequence occurs in the following order:
1 The injection valve switches to the bypass position.
2 The plunger of the metering device moves to the initialization position.
3 The needle lock moves up.
4 The needle moves to the desired sample vial (or well plate) position.
5 The needle lowers into the sample vial (or well plate).
6 The metering device draws the preset sample volume.
7 The needle lifts out of the sample vial (or well plate).
8 The needle is then moved onto the seat to close the sample loop.
9 The needle lock moves down.
10 The injection cycle is completed when the injection valve switches to
the mainpass position.
If needle wash is required it will be done between step 7 and 8.

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